Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463

• Published in: Yeast (Chichester, England) Vol. 23; no. 4; pp. 315 - 323
• Main Authors: Jadhav, J. P., Govindwar, S. P.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.03.2006
• Subjects: Aminopyrine N-Demethylase - metabolism; biodegradation; Biodegradation, Environmental; biosorption; biotransformation; Chromatography, High Pressure Liquid; Laccase - metabolism; leucomalachite green; malachite green; MG reductase; Peroxidases - metabolism; Quinone Reductases - metabolism; Rosaniline Dyes - pharmacokinetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - metabolism; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Water Pollutants, Chemical - pharmacokinetics; yeast
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.1356

In recent years, use of microbial biomass for decolourization of textile industry wastewater is becoming a promising alternative in which some bacteria and fungi are used to replace present treatment processes. Saccharomyces cerevisiae MTCC 463 decolourized the triphenylmethane dyes (malachite green, cotton blue, methyl violet and crystal violet) by biosorption, showing different decolourization patterns. However, malachite green decolourized by biosorption at the initial stage and further biodegradation occurred, about 85% in plain distilled water within 7 h, and about 95.5% in 5% glucose medium within 4 h, under aerobic conditions and at room temperature. Decolourization of malachite green depends on various conditions, such as concentration of dye, concentration of cells, composition of medium and agitation. HPLC, UV‐VIS, FTIR and TLC analysis of samples extracted with ethyl acetate from decolourized culture flasks confirmed the biodegradation of malachite green into several metabolites. A study of the enzymes responsible for the biodegradation of malachite green in the control and cells obtained after decolourization showed the activities of laccase, lignin peroxidase, NADH‐DCIP reductase, malachite green reductase and aminopyrine N‐demethylase in control cells. A significant increase in the activities of NADH‐DCIP reductase and MG reductase was observed in the cells obtained after decolourization, indicating a major involvement of reductases in malachite green degradation. Copyright © 2006 John Wiley & Sons, Ltd.