Confrontation of developing melanophore bars of dark and white axolotls with endogenous dark-embryo mannose-binding lectin correlates with melanophore bar disruption

Lectins are carbohydrate-binding proteins suggested to be important in embryonic cell adhesion/differentiation. Dark and white axolotls contain an endogenous mannose-binding lectin that is especially prevalent during larval melanophore pattern formation (Martha et al., 1990). To determine if this le...

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Bibliographic Details
Published inPigment cell research Vol. 8; no. 2; p. 66
Main Authors Martha, G N, Milos, N C
Format Journal Article
LanguageEnglish
Published Denmark 01.04.1995
Subjects
Ambystoma - embryology
Animals
Deoxyglucose - pharmacology
Lectins - pharmacology
Mannose - pharmacology
Melanophores - physiology
Melanophores - ultrastructure
Pigmentation
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Summary:Lectins are carbohydrate-binding proteins suggested to be important in embryonic cell adhesion/differentiation. Dark and white axolotls contain an endogenous mannose-binding lectin that is especially prevalent during larval melanophore pattern formation (Martha et al., 1990). To determine if this lectin can alter melanophore patterning, lectin extracts have been isolated from Dark embryos by affinity chromatography. The main protein band is 44K on SDS-PAGE. Dark and white embryos at the early chromatophore migration stage have been confronted with Dark lectin or its nonmetabolized inhibitor, 2-deoxyglucose (2-DG). The barred melanophore pattern of both genotypes is disrupted by lectin or 2-DG treatment suggesting that endogenous mannose-binding lectin and its receptor participate in bar formation.
ISSN:0893-5785
DOI:10.1111/j.1600-0749.1995.tb00644.x