Comparison of two commercially available single radial immunodiffusion kits for quantitation of llama immunoglobulin G

• Published in: Journal of veterinary diagnostic investigation Vol. 7; no. 4; p. 515
• Main Authors: Hutchison, J M, Salman, M D, Garry, F B, Johnson, L W, Collins, J K, Keefe, T J
• Format: Journal Article
• Language: English
• Published: United States 01.10.1995
• Subjects: Animals; blood plasma; Camelids, New World - blood; Camelids, New World - immunology; gamma-Globulins - analysis; Immunodiffusion - methods; immunodiffusion tests; Immunoglobulin G - blood; immunoglobuline; immunoglobulins; Indicators and Reagents; inmunoglobulina; lama; llama; llamas; plasma sanguin; plasma sanguineo; Reagent Kits, Diagnostic; Reproducibility of Results; technique d' immunodiffusion; tecnicas de inmunodifusion
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/104063879500700416

Immunoglobin G (IgG) quantitation was performed by a commercially available single radial immunodiffusion kit on 528 plasma samples obtained from llamas. Fifty samples encompassing the range of values obtained were selected for further evaluation. The IgG concentration of these samples was measured by a second commercially available single radial immunodiffusion kit and a sodium sulfite precipitation kit. gamma-Globulins also were measured. IgG values predicted by the single radial immunodiffusion kits were not in agreement. Failure to appreciate this lack of agreement could result in misinterpretation of a llama's IgG level derived from one kit when clinical guidelines established using the other kit are followed.