Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo

• Published in: FEMS immunology and medical microbiology Vol. 18; no. 3; pp. 185 - 192
• Main Authors: Ewen, Stanley W.B., Naughton, Patrick J., Grant, George, Sojka, Marcjanna, Allen-Vercoe, Emma, Bardocz, Susan, Thorns, Christopher J., Pusztai, Arpad
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.1997; Blackwell; Oxford University Press
• Subjects: Animals; Bacteria; Bacteriology; Biological and medical sciences; Biotin; Epithelium; Fimbriae, Bacterial - metabolism; Fundamental and applied biological sciences. Psychology; Ileum; Male; Microbiology; Oral administration; Oral infection; Paraffin; Paraffin wax; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pili; Rabbits; Rat; Rats; Salmonella; Salmonella enterica; Salmonella enterica enteritidis; Salmonella enterica typhimurium; Salmonella enterica var Enteritidis; Salmonella enterica var Typhimurium; Salmonella enteritidis - metabolism; Salmonella typhimurium - metabolism; Streptavidin; Type 1 fimbriae; var Typhimurium; var Enteritidis; Rat; Type 1 fimbriae; Salmonella enteritidis; Digestive system; Rodentia; Pilus; Epithelium; Gene expression; Small intestine; Adhesion; Ileum; Salmonella typhimurium; In vivo; Vertebrata; Mammalia; Infectivity; Bacteria; Enterobacteriaceae
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.1997.tb01044.x

Abstract In a series of experiments rats were dosed with purified type 1 fimbriae from Salmonella enterica var Enteritidis or with fimbriated cultures of either S. enterica var Typhimurium or S. enterica var Enteritidis. Paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sABC) staining technique for the detection of salmonella and type 1 fimbriae. On oral infection with Enteritidis and Typhimurium both bacteria were shown to be closely associated with the rat ileal epithelium and expressed type 1 fimbriae, thus clearly demonstrating that type 1 fimbriae are expressed by salmonellae in vivo. Moreover, association with the ileum was also shown to occur when purified type 1 fimbriae were orally administered to rats. Our results suggest that type 1 fimbriae alone or in combination with other fimbriae may play an important role in the early stages of infection with these pathogenic bacteria.