Facile One‐Pot Three Component Synthesis, Characterization, and Molecular Docking Simulations of Novel α‐Aminophosphonate Derivatives Based Pyrazole Moiety as Potential Antimicrobial Agent

• Published in: Chemistry & biodiversity Vol. 20; no. 10; pp. e202301035 - n/a
• Main Authors: Noser, Ahmed A., Ibrahim, Seham A., Saad‐Allah, Khalil M., Salem, Maha M., Baren, Mohamed H.
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 01.10.2023
• Subjects: Aldehydes; Antimicrobial agents; Antioxidants; Biocides; Catalysts; Chemical synthesis; exo-1,3-β-glucanase; Lithium; Lithium perchlorates; Low concentrations; Membrane proteins; Minimum inhibitory concentration; Mode of action; Molecular docking; outer membrane protein A; Perchlorate; Perchloric acid; Pharmacokinetics; Protein A; Pyrazole; Pyrazoles; Scavenging; three-component reaction; α-aminophosphonates
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202301035

An efficient method has been developed for the synthesis of novel α‐aminophosphonates (AAP) (3 a–m) through a one‐pot three‐component reaction of 1,3‐disubstituted‐1H‐pyrazol‐5‐amine, aromatic aldehydes, and phosphite using lithium perchlorate as catalyst. All newly synthesized compounds were characterized via different spectroscopic techniques. The synthesized compounds′ mode of action was investigated using molecular docking against the outer membrane protein A (OMPA) and exo‐1,3‐β‐glucanase, with interpreting their pharmacokinetics aspects. The results of the antimicrobial effectiveness of these compounds revealed a broad spectrum of their biocidal activity and this in‐vitro study was in line with the in‐ silico results. Additionally, it has been demonstrated that these compounds exhibited a minimum inhibitory concentration (MIC) with significant activity at low concentrations (7.5–30.0 mg/mL). Further, the radical scavenging (DPPH*) activity of the synthesized compounds fluctuated, with compounds 3 h, 3 a, and 3 f showing the highest antioxidant activity. Overall, the formulated compounds can be employed as antimicrobial and antioxidant agents in medical applications.