Synergistic Lysozyme‐Photodynamic Therapy Against Resistant Bacteria based on an Intelligent Upconversion Nanoplatform

• Published in: Angewandte Chemie International Edition Vol. 60; no. 35; pp. 19201 - 19206
• Main Authors: Li, Zhuo, Lu, Shan, Liu, Wenzhen, Dai, Tao, Ke, Jianxi, Li, Xingjun, Li, Renfu, Zhang, Yuxiang, Chen, Zhuo, Chen, Xueyuan
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 23.08.2021
• Edition: International ed. in English
• Subjects: antibacterial agents; Antiinfectives and antibacterials; Bacteria; Bacterial diseases; Bacteriostasis; enzymes; Lysozyme; mesoporous materials; Methicillin; Nanomaterials; Nanoparticles; Nanotechnology; Penetration resistance; Photodynamic therapy; Upconversion; upconversion nanoparticles
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.202103943

The rapid emergence of drug‐resistant bacteria has raised a great social concern together with the impetus for exploring advanced antibacterial ways. NIR‐triggered antimicrobial photodynamic therapy (PDT) by lanthanide‐doped upconversion nanoparticles (UCNP) as energy donor exhibits the advantages of high tissue penetration, broad antibacterial spectrum and less acquired resistance, but is still limited by its low efficacy. Now we designed a bio‐inorganic nanohybrid and combined lysozyme (LYZ) with UCNP‐PDT system to enhance the efficiency against resistant bacteria. Benefiting from the rapid adhesion to bacteria, intelligently bacteria‐responsive LYZ release and synergistic LYZ‐PDT effect, the nanoplatform achieves an exceptionally strong bactericidal capacity and conspicuous bacteriostasis on methicillin‐resistant S. aureus. These findings pave the way for designing efficiently antibacterial nanomaterials and provide a new strategy for combating deep‐tissue bacterial infection. A nanohybrid UCMB‐LYZ‐HP was designed with features of intelligently bacteria‐responsive release of lysozyme and NIR‐triggered high production of 1O2. By synergistically combining the enzymatic and photodynamic effect, the nanohybrid exhibited an extraordinary antibacterial capacity both in vitro and in vivo, providing a promising strategy for combating deep‐tissue infections caused by resistant bacteria.