Cloning of mRNA sequences from the human colon: Preliminary characterisation of defined mRNAs in normal and neoplastic tissues

RNA has been extracted from the normal human colon, converted into cDNA and cloned in the bacterial plasmid pBR322. About 4,000 sequences from this library were screened with probes derived from normal mucosa, familial polyposis mucosa, colonic adenocarcinomas and the colon tumour cell line HT29. So...

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Bibliographic Details
Published inBritish journal of cancer Vol. 54; no. 5; pp. 791 - 798
Main Authors Bartsch, R A, Joannou, C, Talbot, I C, Bailey, D S
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.11.1986
Subjects
Base Sequence
Cell Line
Cloning, Molecular
Colon - analysis
Colonic Neoplasms - pathology
DNA - biosynthesis
Electrophoresis, Agar Gel
Humans
Intestinal Mucosa - pathology
Precancerous Conditions - pathology
RNA, Messenger
Transcription, Genetic
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Summary:RNA has been extracted from the normal human colon, converted into cDNA and cloned in the bacterial plasmid pBR322. About 4,000 sequences from this library were screened with probes derived from normal mucosa, familial polyposis mucosa, colonic adenocarcinomas and the colon tumour cell line HT29. Some mucosal sequences showed greatly reduced levels of transcription in neoplastic conditions, while a few showed elevated transcription. These have been further characterised by Northern and RNA dot-blot analysis.
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ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1986.242