STENOFOLIA gene and regulation of somatic embryogenesis in Medicago truncatula
Expression of STENOFOLIA (STF) gene was analyzed in different organs of line R-108 barrel medic plants ( Medicago truncatula Gaertn.). Maximum levels of STF expression were observed in the generative organs, fruits and flowers, suggesting its involvement in the reproduction process. The local analys...
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Published in | Russian journal of plant physiology Vol. 63; no. 6; pp. 811 - 821 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.11.2016
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Expression of
STENOFOLIA (STF)
gene was analyzed in different organs of line R-108 barrel medic plants (
Medicago truncatula
Gaertn.). Maximum levels of
STF
expression were observed in the generative organs, fruits and flowers, suggesting its involvement in the reproduction process. The local analysis of
STF
expression during somatic embryogenesis in
M. truncatula
showed that the
STF
promoter is activated directly in somatic embryos. Overexpression of
STF
gene in the calli of R-108 line leads to increased embryogenicity, accompanied by reduced levels of
MtGH3.6
gene expression, which is responsible for auxin metabolism, and also reduced expression levels of gene encoding MtHB1 transcription factor. Comparative analysis of
MtGH3.6
and
MtHB1
gene expression dynamics in the calli of embryogenic 2HA line and non-embryogenic A17 line of
M. truncatula
showed that these genes demonstrated reduced level of expression in embryogenic calli compared to non-embryogenic. These data suggested that
STF
stimulates the formation of somatic embryos, directly or indirectly inhibiting the transcription of
MtGH3.6
,
MtHB1
and, probably, many other genes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1021-4437 1608-3407 |
DOI: | 10.1134/S1021443716060133 |