Determination of atrazine, desethyl atrazine and desisopropyl atrazine in environmental water samples using hollow fiber-protected liquid-phase microextraction and high performance liquid chromatography

• Published in: Mikrochimica acta (1966) Vol. 158; no. 1-2; pp. 181 - 186
• Main Authors: Peng, Jinfeng, Lu, Jianxia, Hu, Xialin, Liu, Jingfu, Jiang, Guibin
• Format: Journal Article
• Language: English
• Published: Wien Springer 01.04.2007; New York, NY
• Subjects: Analysis methods; Analytical chemistry; Applied sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Global environmental pollution; Natural water pollution; Other chromatographic methods; Pollution; Water treatment and pollution; Porous membrane; Octanol; Stirring; Microanalysis; HPLC chromatography; Immobilization; Fluorides; Liquid phase; desethyl atrazine; Waste water; desisopropyl atrazine; Hydrochloric acid; Sample preparation; Detection limit; Hollow fiber; Simultaneous measurement; Microextraction; Environment; high-performance liquid chromatography; Standard deviation; Hollow fiber-protected liquid-phase microextraction; Humic acid; Atrazine; Ground water
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-006-0692-9

A new method based on hollow fiber-protected liquid-phase microextraction (LPME) was developed for the simultaneous determination of atrazine, desethyl atrazine and desisopropyl atrazine in environmental water samples. In LPME, analytes were extracted into 1-octanol immobilized in the micropores of a poly(vinylidene fluoride) porous hollow fiber membrane, and back extracted into the acceptor (4M HCl) filled in the lumen of the hollow fiber. After LPME, the analytes trapped in the acceptor were analyzed with high-performance liquid chromatography after neutralization. The effect of extraction factors such as sample pH, acceptor pH, salinity, extraction time, stirring rate, and humic acid were studied. Under the optimized conditions, the limits of detection and relative standard deviations were respectively in the range of 0.5-1.0mugL and 3.9-4.7% (n = 5). The proposed method was applied to determine atrazine, desethyl atrazine and desisopropyl atrazine in wastewater and groundwater samples. The three analytes were below the limits of detection, but good relative spiked recoveries over 90.1 +/- 5.9% at 5mugL spiked level were obtained.