Proteomic analysis of exhaled breath condensate for diagnostics of respiratory system diseases

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 10; no. 3; pp. 230 - 234
• Main Authors: Kononikhin, A. S., Fedorchenko, K. Yu, Ryabokon, A. M., Starodubtseva, N. L., Popov, I. A., Zavialova, M. G., Anaev, E. C., Chuchalin, A. G., Varfolomeev, S. D., Nikolaev, E. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2016; Springer Nature B.V
• Subjects: Biochemistry; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Chronic obstructive pulmonary disease; Mass spectrometry; Medicinal Chemistry; Pneumonia; Proteomics; mass spectrometry; proteomics; pneumonia; chronic obstructive pulmonary disease (COPD); exhaled breath condensate
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750816030069

Study of proteomic composition of exhaled breath condensate (EBC) is a promising non-invasive method for diagnostics of respiratory system diseases in patients. In this study the EBC proteomic composition of 53 donors, including patients with different respiratory system diseases has been investigated. Cytoskeletal keratins type II (1, 2, 3, 4, 5, 6) and cytoskeletal keratins type I (9, 10, 14, 15, 16) were invariant for all samples. Analyzing the frequency of occurrence of proteins in different groups of examined patients, several categories of proteins have been recognized: proteins found in all pathologies (dermcidin, alpha-1- microglobulin, SHROOM3), proteins simultaneously found in two groups (CSTA, LCN1, JUP, PIP, TXN), and proteins specific for a particular group (PRDX1, annexin A1/A2). The EBC analysis by HPLC-MS/MS can be used for identification of potential protein markers specific for inflammatory pulmonary diseases of infective origin (pneumonia) as well as for non-infectious diseases such as chronic obstructive pulmonary disease (COPD).