Physiological and molecular characterization of the late ripening stages in Mangifera indica cv Keitt

• Published in: Postharvest biology and technology Vol. 183; p. 111746
• Main Authors: Busatto, Nicola, Vittani, Lorenzo, Farneti, Brian, Khomenko, Iuliia, Caffini, Matteo, Faccini, Simone, Boschetti, Marco, Costa, Fabrizio
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.2022; Elsevier BV
• Subjects: Biosynthesis; Carotenoids; Cell walls; Commercial shelf life; DA-meter; Ethylene; Fruits; Gene expression; Genes; Mangifera indica; Mangoes; Marketability; Metabolism; Nondestructive testing; Physiology; Post-climacteric ripening; qRT-pcr; Ripening; Senescence; Shelf life; Softening; Spectroscopy; Commercial shelf life; qRT-pcr; Mangifera indica; Ethylene; DA-meter; Softening; Post-climacteric ripening
• ISSN: 0925-5214; 1873-2356
• DOI: 10.1016/j.postharvbio.2021.111746

•Specific molecular regulation characterizes mango post-climacteric ripening.•Senescence phase is coordinated by the expression of ACS and ACO.•Differential activation of cell wall-related genes determines the pulp softening.•Dramatic loss of firmness is the major change during mango post-harvest ripening. Mango is heavily affected by rapid off-tree ripening progression with an excessive fruit softening that eventually limits its marketability. The comprehension of the physiological events occurring in the post-climacteric/senescent phase can contribute to the identification of possible solutions for the improvement of shelf life. In this study, the ripening process of mangoes cv Keitt was monitored for two weeks in a simulated commercial shelf life, after shipping from Brazil to Italy, measuring both ripening-associated parameters (firmness, soluble solid content, ethylene production) and non-destructive method based on Vis/NIR spectroscopy (the ripening index IAD). Moreover, the expression pattern of thirteen genes related to different ripening aspects such as ethylene biosynthesis (ACS, ACO), perception (ETR, ERS) and signaling (EIN2, ERF), in combination with genes responsible for cell wall metabolism (PG14, PG21, EXP, PEL, CEL) and carotenoids accumulation (PSY, NCED) were assessed. The results highlighted a specific gene signature characterizing the post-climacteric softening and the senescence onset. Moreover, the non-destructive IAD has proven to be an effective non-destructive monitoring system of the fruit ripening in mango.