Synthesis of Chlorogenic Acid Imprinted Chromatographic Packing by Surface-initiated Atom Transfer Radical Polymerization and Its Application

• Published in: Chemical research in Chinese universities Vol. 30; no. 5; pp. 855 - 862
• Main Authors: Niu, Yuling, Ma, Meihua, Gong, Yanru, Wang, Yue, Gong, Bolin
• Format: Journal Article
• Language: English
• Published: Changchun Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01.10.2014; Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, P.R.China
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Inorganic Chemistry; Langmuir模型; Organic Chemistry; Physical Chemistry; 傅立叶变换红外光谱仪; 分子印迹聚合物; 原子转移自由基聚合; 引发; 绿原酸; 色谱填料; 表面; Honeysuckle; Chlorogenic acid; Molecularly imprinted chromatographic packing; Surface initiated atom transfer radical polymerization
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-014-4201-0

A novel chromatographic packing of chlorogenic acid（CGA） molecularly imprinted polymer（MIP） based on the 5.0 ~tm silica was prepared by surface initiated atom transfer radical polymerization（SI-ATRP） with 4-vinylpyridine（4-VP） as functional monomer, ethyl glycol dimethacrylate（EDMA） as cross-linker in the mixture of methanol and water（7：3, volume ratio） under mild reaction conditions. The characteristics of CGA MIP were investi- gated by elemental analysis, thermogravimetric analysis（TGA）, Fourier transform infrared spectrometry（FTIR） and atomic force microscopy（AFM）. The effects of some chromatographic conditions such as mobile phase composition and temperature on the retention time were investigated. The adsorption capacity of the stationary phase for com- pounds was determined by frontal chromatographic technique. The results show that Freundlich isotherm fits the ex- perimental adsorption isotherm data better than Langmuir model does. The relatively high heterogeneity index values regressed with the Freundlich isotherm suggest the formation of fairly homogeneous MIP. Thermodynamic data（AAH and AAS） obtained by van＇t Hoff plots reveal an entropy-controlled separation. The CGA MIP column was shown to be successful for the separation and purification of chlorogenic acid from the extract of Honeysuckle.