The effects of a diet with high fat content from lard on the health and adipose-markers’ mRNA expression in mice

• Published in: Science progress (1916) Vol. 107; no. 3; p. 368504241269431
• Main Authors: Chu, Dinh-Toi, Thi, Hue Vu, Bui, Nhat-Le, Le, Ngoc-Hoan
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.07.2024; Sage Publications Ltd
• Subjects: Adipose Tissue - metabolism; Adipose Tissue, White - metabolism; Animal models; Animals; Biology & Life Sciences; Biomarkers; Biomarkers - blood; Biomarkers - metabolism; Blood; Blood levels; Body fat; Body Weight; Body weight gain; Cholesterol; Diet; Diet, High-Fat - adverse effects; Dietary Fats - metabolism; Dyslipidemia; Gene expression; Glucose; Glucose tolerance; High fat diet; Insulin Resistance; Lipogenesis; Male; Mice; Mice, Inbred C57BL; RNA, Messenger - genetics; RNA, Messenger - metabolism; Triglycerides; Triglycerides - blood; Triglycerides - metabolism; Weight Gain; blood dyslipidemia; lard; weight gains; white fat; glucose tolerance; adipose-markers; High fat diet; mRNA expression
• ISSN: 0036-8504; 2047-7163; 2047-7163
• DOI: 10.1177/00368504241269431

Pork is one type of the most frequently consumed meat with about 30% globally. Thus, the questions regarding to the health effects of diet with high fat content from lard are raised. Here, we developed a model of mice fed with high fat (HF) from lard to investigate and have more insights on the effects of long-time feeding with HF on health. The results showed that 66 days on HF induced a significant gain in the body weight of mice, and this weight gain was associated to the deposits in the white fat, but not brown fat. The glucose tolerance, not insulin resistance, in mice was decreased by the HF diet, and this was accompanied with significantly higher blood levels of total cholesterol and triglycerides. Furthermore, the weight gains in mice fed with HF seemed to link to increased mRNA levels of adipose biomarkers in lipogenesis, including Acly and Acaca genes, in white fat tissues. Thus, our study shows that a diet with high fat from lard induced the increase in body weight, white fat depots’ expansion, disruption of glucose tolerance, blood dyslipidemia, and seemed to start affecting the mRNA expression of some adipose biomarkers in a murine model.