Novel Green Fluorescent Probe Stem From Carbon Quantum Dots for Specific Recognition of Tyrosinase in Serum and Living Cells

• Published in: Journal of fluorescence Vol. 33; no. 2; pp. 739 - 750
• Main Authors: Wang, Wenxian, Yang, Dan, Zhou, Yufei, Zhang, Yuding, Guan, Lijiao, Zhang, Xianfen, Xue, Weiming, Huang, Saipeng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2023; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemistry; Biocompatibility; Biological and Medical Physics; Biomarkers; Biomedical and Life Sciences; Biomedical materials; Biomedicine; Biophysics; Biotechnology; Carbon; Catechol; Cell Survival; Citric acid; Fluorescent Dyes; Fluorescent indicators; Humans; Melanoma; Monophenol Monooxygenase; Quantum Dots; Tyrosinase; Fluorescence; Biomarker; Carbon quantum dots; Cell imaging; Tyrosinase activity detection; One-step hydrothermal synthesis
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-022-03101-2

Tyrosinase (TYR), an important biomarker for melanoma, offered significant information early detection of melanoma and may decrease the likelihood of mortality. Therefore, this article constructed a highly sensitive and selective green fluorescent functionalized carbon quantum dots (TYR-CQDs) for tyrosinase (TYR) activity detection by one-step hydrothermal protocol utilizing catechol, citric acid and urea as precursors. The prepared TYR-CQDs illustrated excellent linear relationship and broad linear range with a low detection limit, which exhibited high accuracy and recovery in quantitative determination of TYR in human serum samples. Furthermore, the TYR-CQDs had successfully realized intracellular TYR detection owing to excellent biocompatibility, high anti-interference ability and good cellular imaging capability, suggesting the potential biomedical applications in early diagnosis of melanoma and other tyrosinase-related diseases. Graphical Abstract