HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

• Published in: Chemical research in Chinese universities Vol. 31; no. 1; pp. 38 - 43
• Main Authors: Wang, Hui, Wang, Jiayuan, Wu, Xiaopeng, Shen, Haiyan, Luo, Ying, Dai, Haofu, Mei, Wenli
• Format: Journal Article
• Language: English
• Published: Heidelberg Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01.02.2015; Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine,Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; ESI-MS分析; HPLC-ESI-MS; Inorganic Chemistry; Organic Chemistry; Physical Chemistry; 生物活性成分; 组织培养; 药物活性; 高效液相色谱; 黄酮类化合物; 龙血树; Tissue culture; Dragon’s blood; High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS); Flavonoid; Dragon's blood; Dracaena cambodiana
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-015-4184-5

Dragon＇s blood is a traditional medicine and used in many countries with different cultures because of its various therapeutic properties. The main bioactive constituents of dragon＇s blood are flavonoids, which exhibit various pharmaceutical activities, such as haemostatic, analgesic, anticoagulant, and antimicrobial activities and so on, and have attracted the attention of researchers on the development of new drugs. However, the formation process of dragon＇s blood in nature is very slow, which cannot meet the demand of pharmaceutical uses. Dracaena cambodiana （D. cambodiana） is one of the resource plants of dragon＇s blood na, 6-benzylaminopurine was added in the medium, then red During the course of tissue culture of D. cambodiasecretion was discovered in the cultured medium. Twelve compounds（1--12）, including 11 flavonoids, were determined via the analysis of constituents of the cultured medium by high performance liquid chromatography-electrospray ionization-mass spectrometry（HPLC-ESI-MS） and the results were compared with that of the standards isolated from dragon＇s blood. Among compounds 1-12, 4,4＇- dihydroxy-2-methoxydihydrochalcone（5）, 4,4＇-dihydroxy-2,6-dimethoxydihydrochalcone（6） and （2S）-7,3＇-dihydroxy- 4＇-methoxyflavane（9） were major compounds of the red secretion, with contents of 15.70, 7.10 and 57.23 mg/L, respectively. Therefore, it is promising that flavonoids from dragon＇s blood can be obtained from the tissue culture of its resource plants for the purpose of drug development.