Renal alpha-adrenoceptors and sodium excretion in the dog

• Published in: American journal of physiology. Renal physiology Vol. 248; no. 1 Pt 2; pp. F128 - F133
• Main Authors: Fildes, R D, Eisner, G M, Calcagno, P L, Jose, P A
• Format: Journal Article
• Language: English
• Published: United States 01.01.1985
• Subjects: Animals; Blood Pressure - drug effects; Dogs - metabolism; Female; Glomerular Filtration Rate - drug effects; Kidney - metabolism; Male; Natriuresis - drug effects; Phentolamine - pharmacology; Receptors, Adrenergic, alpha - physiology; Renal Circulation - drug effects; Sodium - urine; Vascular Resistance - drug effects; Yohimbine - pharmacology
• ISSN: 0002-9513; 1931-857X; 1522-1466
• DOI: 10.1152/ajprenal.1985.248.1.F128

The purpose of this study was to characterize the age-dependent role of alpha-adrenergic activity on renal vascular resistance (RVR) and renal blood flow (RBF) and the role of alpha 2-receptors in sodium transport. Intrarenal infusion of phentolamine in hydropenic anesthetized pups and adult dogs increased RBF and glomerular filtration rate (GFR) only in pups. The effect was most marked in the youngest pups (15.67 +/- 0.67 days). At 1.0 micrograms X kg-1 X min-1, phentolamine increased RBF from 1.38 +/- 0.04 to 1.64 +/- 0.04 ml X min-1 X g kidney wt-1 and GFR from 0.19 +/- 0.01 to 0.23 +/- 0.01 ml X min-1 X g kidney wt-1. Absolute (UNaV) and fractional (FENa) sodium excretions increased in all animals, but mean percent increases were greatest in adult dogs. Intrarenal yohimbine infusion in adult dogs (10-100 micrograms X kg-1 X min-1) produced a dose-related increase in UNaV and FENa without affecting RBF and GFR. UNaV increased from 0.22 +/- 0.05 to 0.54 +/- 0.12 mueq X min-1 X g kidney wt-1 and FENa increased from 0.32 +/- 0.05 to 0.63 +/- 0.06% at the dose of 100 micrograms X kg-1 X min-1. These studies confirm a modest role for alpha-adrenoreceptors in the high RVR characteristic of newborn pups and provide evidence for a role of alpha 2-adrenoceptors in the renal transport of sodium; the extent of the contribution of renal alpha-adrenergic system could not be tested in this experiment.