Immunochemical characterization of saline-extracted antigens of Mycobacterium tuberculosis H37Ra

The saline extract (SE) of Mycobacterium tuberculosis H37Ra yielded four major fractions on Sephadex G-100, with average molecular weights of 48,000 (fraction I, F I) 32,000 (fraction II, F II), 15,000 (fraction III, F III) and 5,000 (fraction IV, F IV). FI and FII gave single bands on sodium dodecy...

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Bibliographic Details
Published inInternational archives of allergy and applied immunology Vol. 80; no. 2; p. 221
Main Authors Chawla, T C, Atreyi, M, Jailkhani, B L
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1986
Subjects
Antigens, Bacterial - isolation & purification
Chromatography, Gel
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Humans
Immunochemistry
Mycobacterium tuberculosis - immunology
Serologic Tests
Sodium Chloride - pharmacology
Tuberculosis - diagnosis
Tuberculosis, Gastrointestinal - diagnosis
Tuberculosis, Gastrointestinal - immunology
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Summary:The saline extract (SE) of Mycobacterium tuberculosis H37Ra yielded four major fractions on Sephadex G-100, with average molecular weights of 48,000 (fraction I, F I) 32,000 (fraction II, F II), 15,000 (fraction III, F III) and 5,000 (fraction IV, F IV). FI and FII gave single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showed complete immunological cross-reactivity with each other and were more potent in reacting with antibodies in patients' (tuberculosis) sera than sonicate, SE, purified protein derivative, F III and F IV when tested in a competition enzyme-linked immunosorbent assay.
ISSN:0020-5915
DOI:10.1159/000234055