Efficacy of methyl thujate in inhibiting Penicillium expansum growth and possible mechanism involved

• Published in: Postharvest biology and technology Vol. 161; p. 111070
• Main Authors: Ma, Danying, Ji, Dongchao, Liu, Jialei, Xu, Yong, Chen, Tong, Tian, Shiping
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.03.2020; Elsevier BV
• Subjects: Cell vitality; Flow cytometry; Fluorescence; Fruits; Fungi; Membrane peroxidation; Membrane potential; Membranes; Mitochondria; Mycelia; Penicillium expansum; Penicillum expansum; Peroxidation; Post-harvest decay; ROS; Virulence; Membrane peroxidation; Cell vitality; Penicillum expansum; ROS
• ISSN: 0925-5214; 1873-2356
• DOI: 10.1016/j.postharvbio.2019.111070

•Methyl thujate inhibited mycelial growth of Penicillium expansum and reduced its virulence on harvested fruit.•ROS accumulation and mitochondrial membrane potential abolishment were induced.•Loss of plasma membrane integrity and cytoplasmic content leakage were detected. Fungal infection causes severe diseases on fruits and vegetables, eventually leading to postharvest loss, and thus it is urgent to explore safe substances to control postharvest diseases and elucidate the underlying antimicrobial mechanisms. Here, it was found that a monoterpenoid substance, methyl thujate, was effective in inhibiting mycelial growth of Penicillium expansum and reducing its virulence on harvested fruit dose-dependently. Moreover, dual-fluorescence staining and flow cytometry assay suggested that methyl thujate significantly decreased cell vitality, impaired membrane integrity, induced ROS accumulation and abolished mitochondrial membrane potential in P. expansum. As a consequence, membrane peroxidation and cytoplasmic content leakages occurred. In summary, methyl thujate could inhibit mycelial growth of P. expansum and reduce its virulence on harvested fruit, which is promising for controlling postharvest decay.