Direct somatic embryogenesis and shoot regeneration from leaves and internodes of Pluchea lanceolata (DC.) C.B. Clarke

• Published in: In vitro cellular & developmental biology. Plant Vol. 55; no. 6; pp. 720 - 724
• Main Authors: Kher, Mafatlal M., Nataraj, M.
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media, LLC 01.12.2019; Springer US; Springer Nature B.V
• Subjects: 2,4-D; Acclimatization; Biomedical and Life Sciences; Butyric acid; Callus; Cell Biology; Developmental Biology; Dichlorophenoxyacetic acid; Embryonic growth stage; Herbal medicine; Indole-3-butyric acid; Leaves; Life Sciences; Medicinal plants; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; Plantlets; Pluchea; Propagation; Regeneration; Shoots; SHORT PROTOCOL COMMUNICATION; Somatic embryogenesis; Thidiazuron; Adventitious shoots; Shoot buds; Somatic embryo
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-019-10016-4

Pluchea lanceolata (DC.) C.B. Clarke is a threatened native medicinal plant. Increasing the propagation of this plant will preserve the wild population and provide material for medicinal use. In vitro and field-collected shoots and leaves were tested for response to 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), for initiation of direct shoot regeneration (DSR), or direct somatic embryogenesis (DSE). Leaves and internodes collected from field-grown plants produced only callus, while in vitroraised shoots exhibited DSR and DSE on Murashige and Skoog (MS) medium with 2,4-D and TDZ. Direct shoot regeneration occurred on medium with TDZ from internode and leaf segments obtained from in vitro-developed shoots. In vitro-grovm shoots were rooted on half-strength MS medium with 2 mg L⁻¹ indole-3-butyric acid and acclimatized. Survival in natural conditions was 62.5% for DSE and 79% for DSR plantlets.