Direct somatic embryogenesis and shoot regeneration from leaves and internodes of Pluchea lanceolata (DC.) C.B. Clarke
Pluchea lanceolata (DC.) C.B. Clarke is a threatened native medicinal plant. Increasing the propagation of this plant will preserve the wild population and provide material for medicinal use. In vitro and field-collected shoots and leaves were tested for response to 2,4-dichlorophenoxyacetic acid (2...
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Published in | In vitro cellular & developmental biology. Plant Vol. 55; no. 6; pp. 720 - 724 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
Springer Science + Business Media, LLC
01.12.2019
Springer US Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Pluchea lanceolata (DC.) C.B. Clarke is a threatened native medicinal plant. Increasing the propagation of this plant will preserve the wild population and provide material for medicinal use. In vitro and field-collected shoots and leaves were tested for response to 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), for initiation of direct shoot regeneration (DSR), or direct somatic embryogenesis (DSE). Leaves and internodes collected from field-grown plants produced only callus, while in vitroraised shoots exhibited DSR and DSE on Murashige and Skoog (MS) medium with 2,4-D and TDZ. Direct shoot regeneration occurred on medium with TDZ from internode and leaf segments obtained from in vitro-developed shoots. In vitro-grovm shoots were rooted on half-strength MS medium with 2 mg L⁻¹ indole-3-butyric acid and acclimatized. Survival in natural conditions was 62.5% for DSE and 79% for DSR plantlets. |
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ISSN: | 1054-5476 1475-2689 |
DOI: | 10.1007/s11627-019-10016-4 |