Chungangia koreensis gen. nov., sp. nov., isolated from marine sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 62; no. Pt 8; pp. 1914 - 1920
• Main Authors: Kim, Wonyong, Traiwan, Jitsopin, Park, Mi-Hak, Jung, Min Young, Oh, Su-Jin, Yoon, Jung-Hoon, Sukhoom, Ampaitip
• Format: Journal Article
• Language: English
• Published: Reading International Union of Microbiological Societies 01.08.2012; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; chemotaxonomy; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; genes; Geologic Sediments - microbiology; glucose; Gram-Positive Bacteria - classification; Gram-Positive Bacteria - genetics; Gram-Positive Bacteria - isolation & purification; Lysinibacillus; marine sediments; Microbial ecology; Microbiology; Molecular Sequence Data; new genus; new species; nucleotide sequences; Peptidoglycan - analysis; peptidoglycans; phosphatidylethanolamines; Phospholipids - analysis; Phylogeny; ribose; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; sequence homology; Sporosarcina; Systematics; Various environments (extraatmospheric space, air, water); South Korea; Marine environment; Bacteria; New genus; Marine sediments; New species; Taxonomy
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.028837-0

A Gram-staining-positive, strictly aerobic, non-spore-forming, rod-shaped bacterial strain, CAU 9163T, was isolated from marine sediment collected in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 30 °C and pH 8.0. In phylogenetic analysis based on 16S rRNA gene sequences, strain CAU 9163T formed a hitherto unknown lineage within the order Bacillales, which contains the genera Planomicrobium, Planococcus, Sporosarcina, Rummeliibacillus, Viridibacillus, Lysinibacillus and Bacillus. The levels of 16S rRNA gene sequence similarity between the novel strain and any established bacterial species were all <95.7 %. The major isoprenoid quinines of strain CAU 9163T were MK-8 (65.2 %) and MK-7 (22.8 %) and the predominant fatty acid was anteiso-C15 : 0. The peptidoglycan was of the A4α type and based on l-Lys-d-Asp. The major whole-cell sugars were ribose and glucose. The polar lipid profile mainly consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified polar lipid. The genomic DNA G+C content of the novel strain was 44.3 mol%. These data were sufficient to differentiate the novel strain from established genera in the phylum Firmicutes. Based on the phenotypic, chemotaxonomic and genotypic evidence, strain CAU 9163T represents a novel species in a new genus for which the name Chungangia koreensis gen. nov., sp. nov. is proposed. The type strain of Chungangia koreensis is 9163T ( = KCTC 13729T = CCUG 59778T).