Augmentation of macrophage antitumor activities and nitric oxide production by oregonin

Oregonin, a diarylheptanoid derivative from Alnus hirsuta Turcz, Betulaceae, was evaluated for its antitumor activity. Oregonin, known to have an antitumor function, and is a novel immunomodulator, which may augment macrophage activity. MTT assays and NO production tests were performed in order to i...

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Bibliographic Details
Published inArchives of pharmacal research Vol. 25; no. 4; pp. 457 - 462
Main Authors Joo, Seong-Soo, Kim, Han-Jun, Kwon, Hee-Seung, Lee, Do-Ik
Format Journal Article
LanguageEnglish
Published Korea (South) 01.08.2002
Subjects
Animals
Antineoplastic Agents, Phytogenic - pharmacology
Cell Separation
Cytotoxicity Tests, Immunologic
Diarylheptanoids - pharmacology
Diarylheptanoids - therapeutic use
In Vitro Techniques
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - metabolism
Male
Mice
Mice, Inbred ICR
Neoplasms - pathology
Neoplasms, Experimental - drug therapy
Nitric Oxide - biosynthesis
Stimulation, Chemical
Tetrazolium Salts
Thiazoles
Tumor Cells, Cultured
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Summary:Oregonin, a diarylheptanoid derivative from Alnus hirsuta Turcz, Betulaceae, was evaluated for its antitumor activity. Oregonin, known to have an antitumor function, and is a novel immunomodulator, which may augment macrophage activity. MTT assays and NO production tests were performed in order to investigate the cytotoxicity of oregonin in tumor cells and to examine its influence on macrophage in detail. In this study, the tumoricidal activity was also evaluated by a MTT assay. The cytotoxicity measurements in the oregonin-treated group both in vitro and in vivo showed a significant difference from that of the control group. In vivo, oregonin significantly increased NO production in a dose-dependent manner, and in vitro, the thioglycolate-induced inflammatory macrophages increased NO production in a dose-dependent manner after incubation. These results suggest that oregonin reacts with both the inflammatory and non-inflammatory macrophages in a similar way.
ISSN:0253-6269
1976-3786
DOI:10.1007/bf02976602