Investigation of non-volatile substances in the Haihong fruit wine and their lipid-lowering effect

• Published in: Food bioscience Vol. 39; p. 100836
• Main Authors: Yang, Hui, Su, Wen, Hua, Junli, Wang, Chuang, Wang, Chaoyun
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2021
• Subjects: Free radicals; Haihong fruit wine; Hyperlipidemia; Molecular structure; Non-volatile substances; Haihong fruit wine; Non-volatile substances; Free radicals; Molecular structure; Hyperlipidemia
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2020.100836

Sixteen kinds of non-volatile fat-soluble substances were isolated from the Haihong fruit wine by organic solvent extraction and chromatographic techniques. The structures of 13 compounds were identified by means of mass spectrometry and nuclear magnetic resonance. The relationship between the pharmacological effects of Haihong fruit wine and its active substances such as hyperoside, rutin, catechin, epicatechin, and β-sitosterol was investigated. Results showed that the contents of hyperin, rutin, catechin, epicatechin, naringin dihydrochalcone, β-sterol and other polyphenol flavonoids in the Haihong fruit wine could significantly improve the lipid metabolism of hyperlipidemic mice, increase high-density lipoprotein, reduce blood viscosity, and significantly prevent platelet aggregation and fat accumulation in the liver of mice. The strong reduction and chelation of phenolic hydroxyl groups in hyperin, catechin and rutin are the essential reasons for the pharmacological effects of the fruit wine. These substances can exert their pharmacological effects by eliminating excess free radicals of ·DPPH, ·OH, and ·O2− and by complexing metal ion catalysts which are conducive to the formation of free radicals. [Display omitted] •Sixteen kinds of non-volatile substances were isolated from the Haihong fruit wine.•The fruit wine can improve lipid metabolism and hemorheology.•Polyphenols and flavonoids in the fruit wine can scavenge free radicals.•The conjugation system is positively correlated with lipid-lowering efficacies.