Molecular characterization of loquat EjAP1 gene in relation to flowering
AP1 / SQUA -like gene plays important roles in the development of inflorescences and flowers. In this study, an APETALA1 ( AP1 ) homologue, EjAP1 , was isolated from loquat ( Eriobotrya japonica Lindl.), an economically important subtropical fruit of the Rosaceae. EjAP1 was sequenced and found to be...
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Published in | Plant growth regulation Vol. 70; no. 3; pp. 287 - 296 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.07.2013
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | AP1
/
SQUA
-like gene plays important roles in the development of inflorescences and flowers. In this study, an
APETALA1
(
AP1
) homologue,
EjAP1
, was isolated from loquat (
Eriobotrya japonica
Lindl.), an economically important subtropical fruit of the Rosaceae.
EjAP1
was sequenced and found to be 3,229 bp in length with 6 introns and 7 exons and encoded 239 amino acids. The deduced amino acid sequence contained the typical CaaX-motif of the
AP1
functional proteins and shared high homology with the other
AP1
genes. Phylogenetic analysis at the amino acid level indicated that
EjAP1
belongs to the
AP1/SQUA
subfamily. Transcriptions of
EjAP1
were detected in inflorescence bud, flower bud and flower, but not in vegetative tissues such as vegetative bud. In floral organs, highest expression of
EjAP1
was detected in sepal compared to lower transcription level in petal and stamen, and no transcription in pistil. When ectopic expressed in
ap1
-
1
mutant of
Arabidopsis
,
EjAP1
can partially complement the mutant mainly in that it can restore the sepal and petal formation of
ap1
-
1
mutant. These results indicated that
EjAP1
gene may play a similar role as
Arabidopsis AP1
in the floral development of loquat. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0167-6903 1573-5087 |
DOI: | 10.1007/s10725-013-9800-0 |