cellulase activity of an extreme thermophile

The carboxymethylcellulase activity concentrated from the extremely thermophilic anaerobe H173 was found to have a pH optimum of 6.5-7.0. The enzyme activity was stabilised by the addition of dithiothreitol and CaCl2.2(H2O) and was very stable at 80 degrees C, retaining 77% of the initial activity a...

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Bibliographic Details
Published inApplied microbiology and biotechnology Vol. 35; no. 2; pp. 270 - 273
Main Authors Hudson, J.A, Morgan, H.W, Daniel, R.M
Format Journal Article
LanguageEnglish
Published 01.05.1991
Subjects
carboxymethylcellulose
cellulases
cellulolytic microorganisms
cellulose
cellulose digestion
endo-1,4-beta-glucanase
enzyme activity
hydrolysis
thermophilic bacteria
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Summary:The carboxymethylcellulase activity concentrated from the extremely thermophilic anaerobe H173 was found to have a pH optimum of 6.5-7.0. The enzyme activity was stabilised by the addition of dithiothreitol and CaCl2.2(H2O) and was very stable at 80 degrees C, retaining 77% of the initial activity after 120 min incubation. At 90 degrees C however, 50% activity remained after 9 min and after 120 min only 3% of the initial activity remained. With the enzyme dissolved in buffer, glucose and cellobiose were formed from the hydrolysis of Avicel. In culture medium the Avicel-solubilising activity was insensitive to the presence of up to 50 mM glucose and showed linear glucose accumulation over a period of days at 70 degrees C. HPLC analysis established that glucose was the major end-product of hydrolysis in the culture broths.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00184700