Association of environmental exposure to chromium with differential DNA methylation: An epigenome-wide study
Previous studies have reported that chromium (Cr)-induced epigenetic alterations and DNA methylation play a vital role in the pathogenesis of diseases induced by chromium exposure. Epigenomic analyses have been limited and mainly focused on occupational chromium exposure; their findings are not gene...
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Published in | Frontiers in genetics Vol. 13; p. 1043486 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
04.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Previous studies have reported that chromium (Cr)-induced epigenetic alterations and DNA methylation play a vital role in the pathogenesis of diseases induced by chromium exposure. Epigenomic analyses have been limited and mainly focused on occupational chromium exposure; their findings are not generalizable to populations with environmental Cr exposure.
We identified the differential methylation of genes and regions to elucidate the mechanisms of toxicity related to environmental chromium exposure. DNA methylation was measured in blood samples collected from individuals in Cr-contaminated (
= 10) and unexposed areas (
= 10) by using the Illumina Infinium HumanMethylation850K array. To evaluate the relationship between chromium levels in urine and CpG methylation at 850 thousand sites, we investigated differentially methylated positions (DMPs) and differentially methylated regions (DMRs) by using linear models and DMRcate method, respectively. The model was adjusted for biologically relevant variables and estimated cell-type compositions.
At the epigenome-wide level, we identified five CpGs [cg20690919 (
0.006), cg00704664 (
0.024), cg10809143 (
0.043), cg27057652 (
0.047), cg05390480 (
0.024)] and one DMR (chr17: 19,648,718-19,648,972), annotated to
genes (
< 0.05) as being significantly associated with log
transformed urinary chromium levels.
Environmental chromium exposure is associated with DNA methylation, and the significant DMPs and DMR being annotated to cause DNA damage and genomic instability were found in this work. Research involving larger samples is required to further explore the epigenetic effect of environmental chromium exposure on health outcomes through DNA methylation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Timothy M. Barrow, University of Sunderland, United Kingdom Edited by: Guoping Li, Affiliated Hospital of Southwest Jiaotong University, China These authors have contributed equally to this work Tianyuan Lu, McGill University, Canada This article was submitted to Epigenomics and Epigenetics, a section of the journal Frontiers in Genetics Sangeetha Vishweswaraiah, Beaumont Health, United States |
ISSN: | 1664-8021 1664-8021 |
DOI: | 10.3389/fgene.2022.1043486 |