A colorimetric aptasensor based on gold nanoparticles for detection of microbial toxins: an alternative approach to conventional methods

• Published in: Analytical and bioanalytical chemistry Vol. 414; no. 24; pp. 7103 - 7122
• Main Author: Geleta, Girma Salale
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2022; Springer; Springer Nature B.V
• Subjects: Algae; Algal toxins; Analysis; Analytical Chemistry; Antibodies; Aptamers; Biochemistry; Biocompatibility; Biosensors; Characterization and Evaluation of Materials; Chemical synthesis; Chemistry; Chemistry and Materials Science; Colorimetry; Contaminants; Detection limits; Disaggregation; Fieldwork; Food contamination; Food Science; Gold; Gold compounds; Hydrogen peroxide; Laboratory Medicine; Methods; Microbial toxins; Microorganisms; Monitoring/Environmental Analysis; Mycotoxins; Nanomaterials; Nanoparticles; Physicochemical properties; Poisons; Public health; Review; Selectivity; Sensors; Surface plasmon resonance; Toxins; Trace levels; Ethiopia; Microbial toxins; Gold nanoparticles (AuNPs); Colorimetric aptasensors; Aptamers
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-022-04227-9

Frequent contamination of foods with microbial toxins produced by microorganisms such as bacteria, fungi, and algae represents an increasing public health problem that requires the development of quick and easy tools to detect them at trace levels. Recently, it has been found that colorimetric detection methods may replace traditional methods in the field because of their ease of use, quick response, ease of manufacture, low cost, and naked-eye visibility. Therefore, it is suitable for fieldwork, especially for work in remote areas of the world. However, the development of colorimetric detection methods with low detection limits is a challenge that limits their wide applicability in the detection of food contaminants. To address these challenges, nanomaterial-based transduction systems are used to construct colorimetric biosensors. For example, gold nanoparticles (AuNPs) provide an excellent platform for the development of colorimetric biosensors because they offer the advantages of easy synthesis, biocompatibility, advanced surface functionality, and adjustable physicochemical properties. The selectivity of the colorimetric biosensor can be achieved by the combination of aptamers and gold nanoparticles, which provides an unprecedented opportunity to detect microbial toxins. Compared to antibodies, aptamers have significant advantages in the analysis of microbial toxins due to their smaller size, higher binding affinity, reproducible chemical synthesis and modification, stability, and specificity. Two colorimetric mechanisms for the detection of microbial toxins based on AuNPs have been described. First, sensors that use the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles can exhibit very strong colors in the visible range because of changes caused by aggregation or disaggregation. Second, the detection mechanism of AuNPs is based on their enzyme mimetic properties and it is possible to construct a colorimetric biosensor based on the 3,3',5,5'-tetramethylbenzidine/Hydrogen peroxide, TMB/H 2 O 2 reaction to detect microbial toxins. Therefore, this review summarizes the recent applications of AuNP-based colorimetric aptasensors for detecting microbial toxins, including bacterial toxins, fungal toxins, and algal toxins focusing on selectivity, sensitivity, and practicality. Finally, the most important current challenges in this field and future research opportunities are discussed. Graphical abstract