Relationship between CsLOX gene expression and C6 and C9 aldehydes during cucumber fruit storage

• Published in: Postharvest biology and technology Vol. 161; p. 111085
• Main Authors: Yang, Yuting, Zhang, Tingting, Wang, Xuewei, Wu, Meiqian, Li, Xvzhen, Guo, Xiao, Fang, Yulin, Chen, Shuxia
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.03.2020; Elsevier BV
• Subjects: Aldehydes; C6/C9; Carbon; CsLOXgene expression; Cucumis sativus L; Fruits; Gene expression; Inbreeding; Linoleic acid; Linolenic acid; Principal components analysis; Storage; Volatile compounds; Volatile compounds; C6/C9; Cucumis sativus L; CsLOXgene expression
• ISSN: 0925-5214; 1873-2356
• DOI: 10.1016/j.postharvbio.2019.111085

•Lower content of total volatile compounds was found in 4 °C than 25 °C in both lines.•C6 was produced more rapidly than that of C9 in both temperatures and both lines.•More linoleic acid was cleaved in 4 °C than 25 °C in both lines.•Different CsLOXs gene expression patterns were shown.•Different type CsLOXs were closely related to different type aldehydes formation. Changes in volatile compounds, the ratio of six-carbon (C6) to nine-carbon (C9) aldehydes, the ratio of linoleic acid to linolenic acid and CsLOX gene expression patterns were investigated in cucumber fruits of two different inbred lines after storage at 25 °C or 4 °C. The content of total volatile compounds in both lines was lower and the peak was later for the fruit stored at 4 °C than for that stored at 25 °C, whereas C6 was produced more rapidly than C9 at both temperatures and in both lines. The amount of linoleic acid cleaved at 4 °C was greater than that cleaved at 25 °C, and (E, Z)-2,6-nonadienal/(E)-2-nonenal content was higher at 25 °C than at 4 °C in both lines. There were different expression patterns for cucumber CsLOX genes at the two storage temperatures in the different lines. Principal component analysis showed that the 9-type CsLOX02 was closely related to the formation of C9 aldehydes and the 13-type CsLOX16, CsLOX17, CsLOX19, and CsLOX20 were closely related to formation of C6 aldehydes. Therefore, the expression of CsLOXs might be responsible for differences in the production of volatile compounds at the different storage temperatures.