Specific primer for detection of wheat

Because wheat contains a protein that can trigger allergies, the presence of wheat must be indicated on food products in Japan. One method for detection of wheat is by PCR. However, in the general detection method, only one primer set is used. In this study, we developed a new primer set for detecti...

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Bibliographic Details
Published inNihon Shokuhin Kagaku Kōgaku kaishi Vol. 54; no. 2
Main Authors Uchino, M.(Tokyo Univ. of Agriculture (Japan)), Masubuchi, N, Kurosawa, Y, Noguchi, T, Takano, K
Format Journal Article
LanguageJapanese
Published 01.01.2007
Subjects
ALERGENOS
ALLERGENE
ALLERGENS
BLE
ENSAYO
PCR
TESTAGE
TESTING
TRIGO
WHEATS
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Summary:Because wheat contains a protein that can trigger allergies, the presence of wheat must be indicated on food products in Japan. One method for detection of wheat is by PCR. However, in the general detection method, only one primer set is used. In this study, we developed a new primer set for detection of wheat to protect against false results. Two wheat primer sets, 1 and 2, were constructed. The PCR product using set 1 showed a band around the same molecular weight not only as wheat and rye-wheat containing an allergen compound, but also rye. Two amplified DNA sequences specific to wheat and rye-wheat, using primer set 2,800 bp and 500 bp, were cloned and the sequences were determined. Two STS primer sets, K800 and K500, were reconstructed based on these sequences. PCR conducted using these sets showed specific bands, 800 bp, 500 bp and 450 bp. These were useful for testing of food products containing wheat. In addition, our results showed that the K500 set was superior to the K800 set.
Bibliography:2007004150
S30
ISSN:1341-027X
DOI:10.3136/nskkk.54.82