Antibiotic activity of bacterial endobionts of basidiomycete fruit bodies

• Published in: Microbiology (New York) Vol. 85; no. 6; pp. 752 - 758
• Main Authors: Efimenko, T. A., Malanicheva, I. A., Vasil’eva, B. F., Glukhova, A. A., Sumarukova, I. G., Boikova, Yu. V., Malkina, N. D., Terekhova, L. P., Efremenkova, O. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.11.2016; Springer Nature B.V
• Subjects: Achromobacter; Antibiotics; Antifungal activity; Antimicrobial activity; Bacillus subtilis; Bacteriology; Basidiomycetes; Biomedical and Life Sciences; Ewingella americana; Experimental Articles; Hafnia; Life Sciences; Medical Microbiology; Microbiology; Micrococcus; Nocardia; Physical characteristics; Pseudomonas; Stenotrophomonas maltophilia; endobionts; basidiomycete fruit bodies; antibiotic activity; bacteria; antibiotic resistance
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261716060084

Bacterial strains (93 isolates) capable of growth on full-strength nutrient media were isolated from 86 fungal fruit bodies collected in the Moscow region. Antimicrobial activity of the endobiont isolates against 12 bacterial and fungal test strains (including drug-resistant ones) was studied in submerged cultures. Most of the strains (84.9%) were found to produce antibiotic compounds with different antimicrobial properties, including antifungal activity in 18.3% of the strains. Morphological characteristics and analysis of the 16S rRNA gene sequences were used to determine the taxonomic position of 16 bacterial strains of the following 10 species: Bacillus subtilis , Ewingella americana , Pseudomonas sp., Stenotrophomonas maltophilia , as well as Achromobacter spanius , B. licheniformis , Hafnia paralvei , Micrococcus terreus , Nocardia coeliaca , and St. rhizophila , which have not been previously known to be endobionts of basidiomycete fruit bodies. Antimicrobial activity of A. spanius , E. americana , H. paralvei , M. terreus , N. coeliaca , and St. rhizophila has not been reported previously. Complex mechanisms of symbiotic relations between fungi and bacteria, including those associated with antibiotic formation, probably developed in the course of co-evolution.