Ligand trapping in epithelial layers and cell cultures

We analyze a stochastic model that describes receptor-mediated ligand trapping in epithelial layers and cell culture assays. In both cases, the problem is reduced to diffusion of a Brownian particle between the partially absorbing and reflective surfaces. We derive an analytical expression for the s...

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Published inBiophysical chemistry Vol. 107; no. 3; pp. 221 - 227
Main Authors Berezhkovskii, Alexander M., Batsilas, Lazaros, Shvartsman, Stanislav Y.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.02.2004
Subjects
Animals
Cell culture
Cell Culture Techniques
Diffusion
Epithelial layer
Epithelium - physiology
Humans
Ligand
Ligands
Mathematics
Models, Theoretical
Receptor
Receptors, Cell Surface - physiology
Cell culture
Epithelial layer
Diffusion
Ligand
Receptor
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Summary:We analyze a stochastic model that describes receptor-mediated ligand trapping in epithelial layers and cell culture assays. In both cases, the problem is reduced to diffusion of a Brownian particle between the partially absorbing and reflective surfaces. We derive an analytical expression for the spatial distribution of the trapping points and identify the domains of applicability of the two limiting regimes. We conclude that a thin layer approximation is applicable for ligand trapping in epithelial layers while a typical cell culture experiment is appropriately described within an infinite layer approximation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:0301-4622
1873-4200
DOI:10.1016/j.bpc.2003.09.006