Aldosterone induction and epithelial distribution of CHIF

CHIF is a recently cloned, corticosteroid-induced gene which evokes K+ channel activity in oocytes (B. Attali, H. Latter, N. Rachamim, and H. Garty. Proc. Natl. Acad. Sci. USA 92: 6092-6096, 1995). To further characterize the possible role of this gene in epithelial ion transport, we have studied it...

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Published inAmerican journal of physiology. Renal physiology Vol. 271; no. 2 Pt 2; pp. F322 - F329
Main Authors Wald, H, Goldstein, O, Asher, C, Yagil, Y, Garty, H
Format Journal Article
LanguageEnglish
Published United States 01.08.1996
Subjects
Aldosterone - pharmacology
Animals
Cells, Cultured
Colon - physiology
Dexamethasone - pharmacology
Epithelium - physiology
Gene Expression Regulation
Intracellular Signaling Peptides and Proteins
Kidney - cytology
Kidney - physiology
Male
Potassium Channels - genetics
Potassium Channels - metabolism
Rats
Rats, Wistar
RNA, Messenger - metabolism
Tissue Distribution
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Summary:CHIF is a recently cloned, corticosteroid-induced gene which evokes K+ channel activity in oocytes (B. Attali, H. Latter, N. Rachamim, and H. Garty. Proc. Natl. Acad. Sci. USA 92: 6092-6096, 1995). To further characterize the possible role of this gene in epithelial ion transport, we have studied its epithelial distribution and hormonal induction. Northern hybridizations indicate that the zonal distribution of CHIF mRNA in kidney is: papilla >>medulla>> cortex. High levels of CHIF were also detected in a primary culture from inner medullary collecting duct (IMCD). Perfusing rats with < 20 nM aldosterone through osmotic minipumps evoked a 22.4 +/- 1.9-fold increase in colonic CHIF. A significant increase was observed 3 h after administrating the corticosteroid, but maximal response was detected only after a 72-h incubation. This response appears to be mineralocorticoid specific; perfusing or injecting rats with maximal doses of dexamethasone did not evoke a further increase in CHIF mRNA. In contrast, high levels of CHIF are expressed in kidney papilla and IMCD primary culture, irrespective of corticosteroid treatment. Thus, like the apical Na+ channel and the H(+)-K(+)-adenosinetriphosphatase, CHIF is mineralocorticoid induced in the colon but constitutively expressed in kidney.
ISSN:0002-9513
1931-857X
1522-1466
DOI:10.1152/ajprenal.1996.271.2.f322