Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization

• Published in: Mass spectrometry letters Vol. 3; no. 1; pp. 15 - 17
• Main Authors: Jaesung Pyo, Heesang Lee, Yujin Park, Jiyeong Jo, Yonghoon Park, Sanggil Choe, Miyoung Lee, Jaesin Lee
• Format: Journal Article
• Language: English
• Published: 한국질량분석학회 01.03.2012; 사단법인 한국질량분석학회
• Subjects: 화학; GC-MS; MTBSTFA; PDE-5 inhibitor; BSTFA; TMS derivatization
• DOI: 10.5478/MSL.2012.3.1.015
• ISSN: 2233-4203

Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl(TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes couldbe identified by GC-MS after the derivatization. Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization. KCI Citation Count: 2