Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization
Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high...
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Published in | Mass spectrometry letters Vol. 3; no. 1; pp. 15 - 17 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
한국질량분석학회
01.03.2012
사단법인 한국질량분석학회 |
Subjects | |
Online Access | Get full text |
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Summary: | Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl(TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes couldbe identified by GC-MS after the derivatization. Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization. KCI Citation Count: 2 |
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Bibliography: | G704-SER000003866.2012.3.1.005 |
ISSN: | 2233-4203 2093-8950 |
DOI: | 10.5478/MSL.2012.3.1.015 |