A glycoprotein rapidly labelled with [14C]glucose present in a murine melanoma cell line

If a murine melanoma cell line PG19 is metabolically labelled with [14C]glucose for a short time, one particular membrane component incorporates more radioactivity than the rest. This is a glycoprotein of about Mr 100,000, which is not preferentially labelled in non-malignant variants of the melanom...

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Bibliographic Details
Published inJournal of cell science Vol. 88; no. 3; pp. 335 - 342
Main Author BRAMWELL, M. E
Format Journal Article
LanguageEnglish
Published Cambridge Company of Biologists 01.10.1987
Subjects
Absorptiometry, Photon
Animals
Biological and medical sciences
Cell Line
Cell metabolism, cell oxidation
Cell physiology
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
Glycoproteins - analysis
Melanoma - analysis
Melanoma - metabolism
Mice
Molecular and cellular biology
Neoplasm Proteins - analysis
Tumor Cells, Cultured
Cell culture
Vertebrata
Mammalia
Cell line
Radiolabelling
Mouse
Rodentia
Biosynthesis
Glycoproteins
Glucose
Tumor cell
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Summary:If a murine melanoma cell line PG19 is metabolically labelled with [14C]glucose for a short time, one particular membrane component incorporates more radioactivity than the rest. This is a glycoprotein of about Mr 100,000, which is not preferentially labelled in non-malignant variants of the melanoma or in suppressed hybrid cells formed between PG19 and mouse fibroblasts.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.88.3.335