Chitosan nanoparticles targeted to the tumor-associated ganglioside GD2

Methodological approaches to the creation of nanoparticles based on chitosan derivatives and targeted to the GD2-positive tumor cells were developed. The GD2-specific monoclonal antibodies and their Fab-fragments and scFv-fragments were obtained and studied as vector molecules. Various methods for c...

Full description

Saved in:
Bibliographic Details
Published inRussian journal of bioorganic chemistry Vol. 42; no. 5; pp. 532 - 545
Main Authors Zubareva, A. A., Boyko, A. A., Kholodenko, I. V., Rozov, F. N., Larina, M. V., Aliev, T. K., Doronin, I. I., Vishnyakova, P. A., Molotkovskaya, I. M., Kholodenko, R. V.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.09.2016
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Chitosan
Conjugation
Fragments
Life Sciences
Monoclonal antibodies
Nanoparticles
Organic Chemistry
Reagents
Tumors
targeted nanoparticles
cell death
tumor-associated gangliosides
scFv-fragments
ganglioside GD2
monoclonal antibodies
Fab-fragments
Online AccessGet full text

Cover

More Information
Summary:Methodological approaches to the creation of nanoparticles based on chitosan derivatives and targeted to the GD2-positive tumor cells were developed. The GD2-specific monoclonal antibodies and their Fab-fragments and scFv-fragments were obtained and studied as vector molecules. Various methods for covalent conjugation of these molecules to the nanoparticles were also studied. It was shown that site-specific conjugation of scFv-fragments of GD2-specific antibodies to the chitosan nanoparticles by using a reagent BMPS is the optimal approach to create targeted chitosan-based nanoparticles directed to tumor-associated ganglioside GD2.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162016050150