Selective Cyclooxygenase-1 and -2 Inhibitors Each Increase Allergic Inflammation and Airway Hyperresponsiveness in Mice

• Published in: American journal of respiratory and critical care medicine Vol. 165; no. 8; pp. 1154 - 1160
• Main Authors: Stokes Peebles, R., Jr, Hashimoto, Koichi, Morrow, Jason D, Dworski, Ryszard, Collins, Robert D, Hashimoto, Yuko, Christman, John W, Kang, Kyung-Ho, Jarzecka, Kasia, Furlong, Jamye, Mitchell, Daphne B, Talati, Megha, Graham, Barney S, Sheller, James R
• Format: Journal Article
• Language: English
• Published: New York, NY Am Thoracic Soc 15.04.2002; American Lung Association
• Subjects: Allergic diseases; Animals; Arachidonic Acid - metabolism; Biological and medical sciences; Bronchial Hyperreactivity - immunology; Bronchial Hyperreactivity - metabolism; Bronchial Hyperreactivity - physiopathology; Bronchial Provocation Tests; Bronchoalveolar Lavage Fluid - chemistry; Cyclooxygenase 1; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors - pharmacology; Dinoprostone - analysis; Female; Immunization; Immunopathology; Interleukins - metabolism; Isoenzymes - antagonists & inhibitors; Isoenzymes - metabolism; Lung - metabolism; Medical sciences; Membrane Proteins; Methacholine Chloride; Mice; Mice, Inbred BALB C; Organic Chemicals; Ovalbumin - immunology; Prostaglandin-Endoperoxide Synthases - metabolism; Pyrazoles; Receptors, Chemokine - metabolism; Respiratory and ent allergic diseases; Sulfonamides; Immunopathology; Allergy; Prostaglandin-endoperoxide synthase; Respiratory disease; Enzyme; Cytokine; Rodentia; Exploration; Inflammation; Interleukin 13; Biological activity; Asthma; Vertebrata; Mammalia; Interleukin 5; Mouse; Animal; Obstructive pulmonary disease; Oxidoreductases; Inhibition
• ISSN: 1073-449X; 1535-4970
• DOI: 10.1164/ajrccm.165.8.2106025

Nonselective cyclooxygenase (COX) inhibition during allergic sensitization with ovalbumin in a murine model leads to an increase in the Type 2 cytokines interleukin-5 (IL-5) and IL-13; however, the effect of selective COX-1 and COX-2 inhibitors on these cytokines is unknown. We found that COX-1 protein was constitutively expressed in lung tissue. Expression of COX-1 protein did not increase with ovalbumin sensitization, but expression of COX-2 protein did. Ovalbumin-sensitized mice treated with either selective COX-1 inhibitor SC58560 (OVA-COX-1 inhibitor) or selective COX-2 inhibitor SC58236 (OVA-COX-2 inhibitor) had significantly greater airway hyperresponsiveness (p < 0.05) and higher levels of IL-13 (p < 0.05) in lung supernatants than did untreated mice that were ovalbumin sensitized (OVA). Lung mRNA levels for the chemokine receptors CCR1 through CCR5 (expressed on eosinophils, basophils, lymphocytes, and dendritic cells) were increased in the OVA-COX-2 inhibitor and OVA-indomethacin groups. We conclude that in the BALB/c mouse, COX inhibition with either a COX-1 or COX-2 inhibitor during allergen sensitization augments production of IL-13 and increases airway hyperresponsiveness.