Detection of interleukin-8 on microgapped dual electrodes for measuring asthma complication

• Published in: Biotechnology and applied biochemistry Vol. 68; no. 6; p. 1342
• Main Authors: Zhang, Lihong, Dai, Wenqiong, Ren, Ming, Wang, Hui, Niu, Ya, Li, Lihua, Xu, Bei, Wang, Yajuan, Gopinath, Subash C B
• Format: Journal Article
• Language: English
• Published: United States 01.12.2021
• Subjects: Asthma - complications; Asthma - diagnosis; Biosensing Techniques; Electrochemical Techniques; Electrodes; Graphite - chemical synthesis; Graphite - chemistry; Humans; Interleukin-8 - analysis; Surface Properties; allergen; graphene oxide; immunosensor; interleukin; respiratory disease
• ISSN: 1470-8744
• DOI: 10.1002/bab.2056

Detection of asthma by a suitable biomarker is mandatory for the early identification, which helps in providing a right medication for the complete cure. Interleukins (ILs) have played a major role in asthma; in particular IL-8 is highly correlated with severe asthma. This research was focused on to detect IL-8 level by its partner antibody on a microgapped dual electrodes sensor. The sensing surface was modified into graphene oxide (GO), and an antibody was fixed by using the amine-aldehyde linker. GO enhanced the antibody immobilization and the consequence electric current flow upon interacting with IL-8. The detection limit of IL-8 was reached to 10 pg/mL in a linear range from 1 to 10,000 pg/mL with the regression of y = 0.7246x - 0.906 (R² = 0.9758); further, the sensitivity falls at 1 pg/mL. The surface does not show the antifouling effect with control antibody, and proteins, indicating the specific IL-8 detection. The detection of IL-8 helps in diagnosing and solving the related problems of asthmatic patients.