A sensitive fluorescent sensor based on the photoinduced electron transfer mechanism for cefixime and ctDNA

• Published in: Journal of molecular recognition Vol. 33; no. 3; pp. e2816 - n/a
• Main Authors: Yang, Cheng‐Zhang, Liu, Yong‐Chang, Xu, Cheng, Bai, Ai‐Min, Hu, Yan‐Jun
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.03.2020
• Subjects: Antibiotics; Binding; Cadmium tellurides; CdTe:Zn2+ QDs; Cefixime; Cephalosporins; ctDNA; Electrochemical analysis; Electrochemistry; Electron transfer; Fluorescence; fluorescent sensor; Fourier transforms; Infrared spectra; Oral administration; photoinduced electron transfer; Quantum dots; Zinc; cefixime; photoinduced electron transfer; CdTe:Zn2+ QDs; ctDNA; fluorescent sensor
• ISSN: 0952-3499; 1099-1352
• DOI: 10.1002/jmr.2816

Cefixime is a third generation orally administered cephalosporin that is frequently used as a broad spectrum antibiotic against various gram‐negative and gram‐positive bacteria. In this study, a simple and sensitive fluorescent sensor for the determination of the cefixime and ctDNA was established based on the CdTe:Zn2+ quantum dots (QDs). The fluorescence of CdTe:Zn2+ QDs can be effectively quenched by cefixime in virtue of the surface binding of cefixime on CdTe:Zn2+ QDs and the subsequent photoinduced electron transfer process from CdTe:Zn2+ QDs to cefixime, in particular, the high sensitivity of QDs fluorescence emission to cefixime at the micromole per liter level, which render the cefixime‐CdTe:Zn2+ QDs system into fluorescence “OFF” status, then turn on in the presence of ctDNA. Furthermore, the Fourier transform infrared (FTIR) spectra of characteristic bands of C–N and N–H groups of cefixime endow evidence for the interaction of cefixime with CdTe:Zn2+ QDs. The relative electrochemical behavior of the affinity of CdTe:Zn2+ QDs for cefixime and ctDNA reveals the potential molecular binding mechanism. The research of photoinduced electron transfer sensor system based on quantum dots still needs to be greatly developed. In this work, the ingenious introduction of the currently focused cefixime as quenching molecules, the addition of double‐stranded calf thymus DNA leads to the "turn off‐on" transition of CdTe:Zn2+ QDs fluorescence, the optical response recognition process of CdTe:Zn2+ QDs‐Cefixime‐ctDNA is discussed in detail. It can provide a theoretical basis for the development of DNA probe and the detection of antibiotics.