Vatairea guianensis lectin stimulates changes in gene expression and release of TNF‐α from rat peritoneal macrophages via glycoconjugate binding

• Published in: Journal of molecular recognition Vol. 34; no. 10; pp. e2922 - n/a
• Main Authors: Marques, Gabriela Fernandes Oliveira, Pires, Alana Freitas, Osterne, Vinicius Jose Silva, Pinto‐Junior, Vanir Reis, Silva, Ivanice Bezerra, Martins, Maria Gleiciane Queiroz, Oliveira, Messias Vital, Gomes, Angelica Maciel, Souza, Luiz Augusto Gomes, Pavão, Mauro Sérgio Gonçalves, Cavada, Benildo Sousa, Assreuy, Ana Maria Sampaio, Nascimento, Kyria Santiago
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.10.2021
• Subjects: Animal models; Animals; Binding; Cell activation; Cell Movement - drug effects; Cell surface; Cells, Cultured; Disease Models, Animal; Fabaceae - chemistry; Female; Gene expression; Gene Expression Regulation - drug effects; Glycan; glycoconjugates; Glycoconjugates - chemistry; Glycoconjugates - metabolism; In vivo methods and tests; Inflammation; Inflammatory response; Lectins; Leukocyte migration; Leukocytes; Leukocytes (neutrophilic); Leukocytes - drug effects; macrophage; Macrophages; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Molecular docking; Molecular structure; Peritoneum; Peritonitis; Peritonitis - chemically induced; Peritonitis - metabolism; Peritonitis - pathology; Permeability; Plant Lectins - chemistry; Plant Lectins - metabolism; Plant Lectins - pharmacology; Polysaccharides; Rats; Rats, Wistar; Receptors; Receptors, Tumor Necrosis Factor, Type I - chemistry; Receptors, Tumor Necrosis Factor, Type I - metabolism; Seeds; TLR4 protein; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Tumor necrosis factor; Tumor necrosis factor receptor 1; Tumor necrosis factor receptors; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Variance analysis; Vatairea guianensis; Vatairea guianensis; macrophage; inflammation; glycoconjugates; lectins
• ISSN: 0952-3499; 1099-1352
• DOI: 10.1002/jmr.2922

Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll‐like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose‐dependent gene expression and release of TNF‐α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF‐α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin‐induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF‐α gene expression (RT‐PCR) and release from peritoneal macrophages. Thus, upon lectin‐glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF‐α and TLR and/or TNFR receptor pathways. The interaction effects of the Vatairea guianensis lectin on the glycans of TNFR1 (tumor necrosis factor receptor 1) and TRL4 (toll‐like receptor 4) that elicit inflammation.