Serotonin uptake and release from rat cerebellum in vitro
Serotonin (5-HT) uptake and release were analyzed in cerebellar fractions. Cerebellar, P2 fractions and molecular layer homogenates are able to accumulate exogenous 5-HT through an Na-dependent, high-affinity transport system. Molecular layer 5-HT uptake, however, was totally dependent upon Na+, whe...
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Published in | Journal of neuroscience research Vol. 12; no. 1; p. 129 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
1984
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Subjects | |
Online Access | Get more information |
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Summary: | Serotonin (5-HT) uptake and release were analyzed in cerebellar fractions. Cerebellar, P2 fractions and molecular layer homogenates are able to accumulate exogenous 5-HT through an Na-dependent, high-affinity transport system. Molecular layer 5-HT uptake, however, was totally dependent upon Na+, whereas the accumulation of the amine by P2 fractions from whole cerebellum was also carried out by an Na-independent transport system. Ca++-dependent release of recently accumulated 5-HT was observed in both P2 fractions and molecular layer homogenates when depolarizing conditions were used. This release was blocked by ruthenium red and Mg++, two inhibitors of the Ca++ entry to the nerve endings induced by depolarization. The molecular layer homogenates released twice as much 5-HT as did cerebellar P2 fractions, indicating that serotoninergic boutons in the cerebellar cortex might be located in the molecular layer. Our results suggest that 5-HT might play a transmitter role in the cerebellum. |
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ISSN: | 0360-4012 |
DOI: | 10.1002/jnr.490120112 |