Needle biopsy‐derived myofascial tissue samples are sufficient for quantification of myofibroblast density
Quantification of myofibroblasts is a promising method for assessing tissue properties in the field of fascia research. This is commonly performed by immunohistochemistry for α‐smooth muscle actin. However, usually larger tissue samples sizes are required for quantification. The aim of this investig...
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Published in | Clinical anatomy (New York, N.Y.) Vol. 31; no. 3; pp. 368 - 372 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Wiley Subscription Services, Inc
01.04.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Quantification of myofibroblasts is a promising method for assessing tissue properties in the field of fascia research. This is commonly performed by immunohistochemistry for α‐smooth muscle actin. However, usually larger tissue samples sizes are required for quantification. The aim of this investigation was to explore whether a microscopic quantification of myofibroblasts can be conducted with fascial tissue samples derived via percutaneous needle biopsy. Fascial tissues were derived via percutaneous needle biopsy from the fascia lata of 11 persons (aged 19–40 years). Following immunohistochemistry, selected fields for photomicroscopic analysis were chosen by a Monte Carlo method based randomization procedure. On these fields, a digital quantification for the relative density of α‐smooth muscle actin was attempted. The newly developed quantification method could successfully be applied in all tissue samples. The median α‐smooth muscle actin density in the selected tissue samples ranged between 0% and 1.7% (median 0%, IQR 0%–0.001%). The applied protocol proved to be workable for the purpose of an estimation of the α‐smooth muscle actin density in fascial tissue samples derived via percutaneous needle biopsy. Since this type of biopsy is less invasive than the commonly performed open muscle biopsy, this offers a new and useful perspective for future histological investigations of fascial tissue properties in living patients. Clin. Anat. 31:368–372, 2018. © 2018 Wiley Periodicals, Inc. |
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Bibliography: | Dr. Schleip reports funding from the European Rolfing Association e.V. This funding is not related to the current study. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0897-3806 1098-2353 |
DOI: | 10.1002/ca.23040 |