Using xCELLigence RTCA Instrument to Measure Cell Adhesion

Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within...

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Bibliographic Details
Published inBio-protocol Vol. 7; no. 24
Main Authors Hamidi, Hellyeh, Lilja, Johanna, Ivaska, Johanna
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.12.2017
Bio-protocol LLC
Subjects
Biology
Methods
Real-time cell analysis
Extracellular matrix
xCELLigence
Cell adhesion
Integrins
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ISSN2331-8325
2331-8325
DOI10.21769/BioProtoc.2646

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Summary:Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are associated with a variety of human pathologies. Here, we outline a simple protocol to monitor alterations in cell adhesion to the ECM, for example, following genetic manipulations or overexpression of a protein of interest or in response to drug treatment, using the xCELLigence real-time cell analysis (RTCA) system.
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Contributed equally to this work
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2646