Separation of polyphenols from leaves of Malus hupehensis (Pamp.) Rehder by off-line two-dimensional High Speed Counter-Current Chromatography combined with recycling elution mode

• Published in: Food chemistry Vol. 186; pp. 139 - 145
• Main Authors: Liu, Qi, Zeng, Hualiang, Jiang, Shujing, Zhang, Li, Yang, Fuzhu, Chen, Xiaoqing, Yang, Hua
• Format: Journal Article
• Language: English
• Published: England 01.11.2015
• Subjects: Chromatography, High Pressure Liquid; Countercurrent Distribution; Flavonoids - analysis; Glucose - analysis; Hexanes - chemistry; Kaempferols - analysis; Magnetic Resonance Spectroscopy; Malus - chemistry; Monosaccharides - analysis; Phloretin - analysis; Plant Extracts - analysis; Plant Leaves - chemistry; Polyphenols - analysis; Solvents - chemistry; Closed-loop recycling; Malus hupehensis (Pamp.) Rehder; Phlorizin; 3-Hydroxy-phlorizin; Recycling HSCCC
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.09.037

In this study, off-line two-dimensional High Speed Counter-Current Chromatography (2D HSCCC) strategy combined with recycling elution mode was developed to isolate compounds from the ethyl acetate extract of a common green tea--leaves of Malus hupehensis (Pamp.) Rehder. In the orthogonal separation system, a conventional HSCCC was employed for the first dimension and two recycling HSCCCs were used for the second in parallel. Using a solvent system consisting of n-hexane-ethyl acetate-methanol-water (1:4:0.6:4.4, v/v) in the first and second dimension, four compounds including 3-hydroxy-phlorizin (1), phloretin (2), avicularin (3) and kaempferol 3-O-β-D-glucoside (4) were obtained. The purities of these four compounds were all over 95.0% as determined by HPLC. And their structures were all identified through UV, MS and (1)H NMR. It has been demonstrated that the combination of off-line 2D HSCCC with recycling elution mode is an efficient technique to isolate compounds with similar polarities in natural products.