Forced Degradation of Flavonol Glycosides Extraced from Ginkgo biloba

• Published in: Chemical research in Chinese universities Vol. 29; no. 4; pp. 667 - 670
• Main Authors: Jin, Ye, Zhang, Wei-yu, Meng, Qing-fan, Li, Dan-hui, Garg, Sajay, Teng, Li-rong, Wen, Jing-yuan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2013; College of Life Science, Jilin University, Changchun 130012, P.R.China; School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, P.R.China%School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, P.R.China%College of Life Science, Jilin University, Changchun 130012, P.R.China%School of Pharmacy, Faculty of Medical and Health Sciences, University of Auckland, 1010 Auckland, New Zealand%School of Pharmacy and Medical Sciences, University of South Australia, Adelaide,South Australia 5001, Australia
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Inorganic Chemistry; NaOH溶液; Organic Chemistry; Physical Chemistry; RP-HPLC; 反相高效液相色谱法; 磷酸盐缓冲液; 银杏叶提取物; 银杏黄酮苷; 降解产物; 黄酮醇苷; Degradation; pH-Stability; flavonol glycoside; Degradation rate constant; Ginkgo flavonol glycoside
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-013-2352-z

The degradation of flavonol glycosides extracted from Ginkgo biloba was performed under different conditions and the degraded products were determined by reversed-phase high performance liquid chromatography （RP-HPLC） method.Four stress conditions including acid（0.1 mol/L HCl）,base（0.1 mol/L NaOH）,temperature （70 ℃） and oxidation（0.03% H2O2,volume fraction） were used for the forced degradation studies.The pH stabilities of the flavonol glycosides were determined in phosphate buffers of varying pH values from 4.5 to 7.4.The degradation rate constants and half-life of three Ginkgo flavonol aglycones（quercetin,kaempferol and isorhamnetin） which represent Ginkgo flavonol glycosides were calculated in forced degradation and pH-stability studies of them.The resuits indicate that the three substances were more stable when incubated under acid condition and showed pH-dependent stability.The degradation was observed to follow first-order kinetics in all degradation studies.The stability results could provide important bases on development,preparation and storage of products of Ginkgo biloba extract and should be significantly considered during the further formulation development.