Metaproteomics: Sample Preparation and Methodological Considerations

Meta-omic techniques have progressed rapidly in the past decade and are frequently used in microbial ecology to study microorganisms in their natural ecosystems independent from culture restrictions. Metaproteomics, in combination with metagenomics, enables quantitative assessment of expressed prote...

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Published inAdvances in experimental medicine and biology Vol. 1073; p. 187
Main Authors Kunath, Benoit J, Minniti, Giusi, Skaugen, Morten, Hagen, Live H, Vaaje-Kolstad, Gustav, Eijsink, Vincent G H, Pope, Phil B, Arntzen, Magnus Ø
Format Journal Article
LanguageEnglish
Published United States 01.01.2019
Subjects
Metagenomics
Binning
Functional analysis
Microbial communities
Metaproteomics
Assembly
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Summary:Meta-omic techniques have progressed rapidly in the past decade and are frequently used in microbial ecology to study microorganisms in their natural ecosystems independent from culture restrictions. Metaproteomics, in combination with metagenomics, enables quantitative assessment of expressed proteins and pathways from individual members of the consortium. Together, metaproteomics and metagenomics can provide a detailed understanding of which organisms occupy specific metabolic niches, how they interact, and how they utilize nutrients, and these insights can be obtained directly from environmental samples. Here, we outline key aspects of sample preparation, database generation, and other methodological considerations that are required for successful quantitative metaproteomic analyses and we describe case studies on the integration with metagenomics for enhanced functional output.
ISSN:0065-2598
DOI:10.1007/978-3-030-12298-0_8