Agents of Campylobacteriosis in Different Meat Matrices in Brazil

• Published in: International journal of environmental research and public health Vol. 19; no. 10; p. 6087
• Main Authors: Takeuchi, Micaela Guidotti, de Melo, Roberta Torres, Dumont, Carolyne Ferreira, Peixoto, Jéssica Laura Miranda, Ferreira, Gabriella Rayane Aparecida, Chueiri, Mariana Comassio, Iasbeck, Jocasta Rodrigues, Timóteo, Marcela Franco, de Araújo Brum, Bárbara, Rossi, Daise Aparecida
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.05.2022; MDPI
• Subjects: Amoxicillin; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Beef; Biofilms; Brazil - epidemiology; Campylobacter; Campylobacter Infections - epidemiology; Campylobacteriosis; Carcasses; Cattle; Cellulose; Chickens; Ciprofloxacin; Fluoroquinolones; Food industry; Food Microbiology; Food processing; Food processing industry; Gastroenteritis; Genetic diversity; Genotypes; Infections; LuxS protein; Meat; Meat industry; Microbial Sensitivity Tests; Multidrug resistance; Pathogens; Pork; Poultry; Protocol; Strains (organisms); Virulence; Brazil; United States > US; chilled meat; frozen meat; virulence; RAPD; antimicrobial resistance
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph19106087

We aimed to identify the prevalence of thermophilic species of in meats of different species available on the Brazilian commercial market and to determine the genetic diversity, antimicrobial resistance and virulence potential of the isolates. A total of 906 samples, including chicken, beef and pork carcasses and chicken and beef livers, were purchased in retail outlets, and prevalences of 18.7% (46/246), 3.62% (5/138), 10.14% (14/138), 3.62% (5/138) and 4.47% (11/132), respectively, were identified, evidencing the dissemination of genotypes in the main producing macro-regions. Of all isolates, 62.8% were classified as multidrug resistant (MDR), with resistance to amoxicillin-clavulanate (49.4%), tetracycline (51.8%) and ciprofloxacin (50.6%) and co-resistance to macrolides and fluoroquinolones (37.1%). Multivirulent profiles were identified mainly in isolates from chicken carcasses (84.8%), and the emergence of MDR/virulent strains was determined in pork isolates. All isolates except those from chicken carcasses showed a high potential for biofilm formation (71.4% ) and consequent persistence in industrial food processing. For chicken carcasses, the general virulence was higher in (54.3%), followed by (24%) and spp. (21.7%), and in the other meat matrices, spp. showed a higher prevalence of virulence (57.2%). The high rates of resistance and virulence reinforce the existence of strain selection pressure in the country, in addition to the potential risk of strains isolated not only from chicken carcasses, but also from other meat matrices.