Enteral plasma supports brain repair in newborn pigs after birth asphyxia

• Published in: Brain, behavior, and immunity Vol. 119; pp. 693 - 708
• Main Authors: Ventura, Gemma Chavarria, Dyshliuk, Nadiya, Dmytriyeva, Oksana, Nordsten, Mads Jacob Bagi, Haugaard, Maria Mathilde, Christiansen, Line Iadsatian, Thymann, Thomas, Sangild, Per Torp, Pankratova, Stanislava
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2024
• Subjects: Birth asphyxia; Caudate nucleus; Motor cortex; Motor development; Plasma; Plasma; Birth asphyxia; Caudate nucleus; Motor development; Motor cortex
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2024.04.032

•Clamping of the umbilical cord during caesarean delivery of piglets recapitulates moderate birth asphyxia (ASP) of human neonates.•ASP impairs the early development of motor functions.•ASP induces death of CTIP2+-neurons in the motor cortex and caudate and arrests the maturation of oligodendrocyte precursor cells.•Dietary plasma mitigates ASP-induced encephalopathy by induction of anti-inflammatory, anti-oxidative, and neuroprotective mechanisms.•Dietary plasma may be a source of neuroprotective bioactive elements. Newborns exposed to birth asphyxia transiently experience deficient blood flow and a lack of oxygen, potentially inducing hypoxic-ischaemic encephalopathy and subsequent neurological damage. Immunomodulatory components in plasma may dampen these responses. Using caesarean-delivered pigs as a model, we hypothesized that dietary plasma supplementation improves brain outcomes in pigs exposed to birth asphyxia. Mild birth asphyxia was induced by temporary occlusion of the umbilical cord prior to caesarean delivery. Motor development was assessed in asphyxiated (ASP) and control (CON) piglets using neonatal arousal, physical activity and gait test parameters before euthanasia on Day 4. The ASP pigs exhibited increased plasma lactate at birth, deficient motor skills and increased glial fibrillary acidic protein levels in CSF and astrogliosis in the putamen. The expression of genes related to oxidative stress, inflammation and synaptic functions was transiently altered in the motor cortex and caudate nucleus. The number of apoptotic cells among CTIP2-positive neurons in the motor cortex and striatal medium spiny neurons was increased, and maturation of preoligodendrocytes in the internal capsule was delayed. Plasma supplementation improved gait performance in the beam test, attenuated neuronal apoptosis and affected gene expression related to neuroinflammation, neurotransmission and antioxidants (motor cortex, caudate). We present a new clinically relevant animal model of moderate birth asphyxia inducing structural and functional brain damage. The components in plasma that support brain repair remain to be identified but may represent a therapeutic potential for infants and animals after birth asphyxia.