Producing GST-Cbx7 Fusion Proteins from Escherichia coli

This protocol describes the production of GST-Cbx7 fusion proteins from , originally developed in the recent publication (Zhen , 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopropyl-beta-D-thio...

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Bibliographic Details
Published inBio-protocol Vol. 7; no. 12
Main Authors Huynh, Thao Ngoc, Ren, Xiaojun
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.06.2017
Bio-protocol LLC
Subjects
Biology
Methods
Cbx7
pGEX-6P-1
Polycomb
GST-fusion proteins
Glutathione Sepharose 4B
Affinity purification
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Summary:This protocol describes the production of GST-Cbx7 fusion proteins from , originally developed in the recent publication (Zhen , 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B. This protocol can be adapted for the purification of other proteins.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2333