Dissociation of ferriheme from oxidized heme proteins and re-reduction of ferriheme to ferroheme are crucial for the formation of zinc protoporphyrin IX in nitrite/nitrate-free dry-cured meat products

• Published in: Food chemistry Vol. 427; p. 136755
• Main Authors: Zhai, Yang, Abe, Haruka, Wang, Hung-Cheng, Hayakawa, Toru, Kumura, Haruto, Wakamatsu, Jun-ichi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.11.2023
• Subjects: azides; carbon monoxide; cured meats; dissociation; food chemistry; ham; heme; Heme dissociation; Heme reduction; hemoglobin; ligands; nitrates; nitric oxide; Nitrite; nitrites; oxidation; protoporphyrin; zinc; Zinc protoporphyrin IX; Zinc protoporphyrin IX; Heme dissociation; Nitrite; Heme reduction
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2023.136755

•Not ferroheme but ferriheme can be predominantly dissociated from heme proteins.•The strong heme-ligand binding inhibits the iron release of heme and ZnPP formation.•The reduction of the dissociated ferriheme is essential for ZnPP formation. Zinc protoporphyrin IX (ZnPP) is the dominant red pigment in nitrate/nitrite-free dry-cured meat products such as Parma ham, and it is considered to be a potential alternative to nitrite/nitrate for reddening dry-cured meat products. Ferroheme and ferriheme dissociated from heme proteins in meat were proposed as substrates to form ZnPP. To elucidate their specific formation mechanism, nitric oxide, carbon monoxide, and azide were used to stable heme in heme proteins. The exogenous hemoglobin derivatives bound with these ligands showed lower heme dissociation compared with exogenous oxyhemoglobin and did not contribute to ZnPP formation. Meanwhile, azide inhibited almost all ZnPP formation by binding to ferriheme, indicating ferriheme dissociation from oxidized heme proteins, predominantly for ZnPP formation. Free ferriheme could not be converted to ZnPP unless it was reduced to ferroheme. Overall, ferriheme dissociated from oxidized heme proteins was the dominant substrate for conversion to ZnPP after re-reduction to ferroheme.