Differential antioxidation activities in two alfalfa cultivars under chilling stress

• Published in: Plant biotechnology reports Vol. 3; no. 4; pp. 301 - 307
• Main Authors: Wang, Wen-Bin, Kim, Yun-Hee, Lee, Haeng-Soon, Deng, Xi-Ping, Kwak, Sang-Soo
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 01.10.2009; Springer Nature B.V; 한국식물생명공학회
• Subjects: Adaptability; Agriculture; Alfalfa; Antioxidants; Biomedical and Life Sciences; Biotechnology; Cell Biology; Crops; Cultivars; Enzymes; Germination; Hydrogen peroxide; Laboratories; Life Sciences; Lipid peroxidation; Lipids; Original Article; Oxidative stress; Peroxidation; Plant Biochemistry; Plant Sciences; Proteins; Seed germination; Seedlings; Seeds; Soil erosion; Studies; Temperature effects; 농학; Chilling stress; Lipid peroxidation; Alfalfa; Antioxidant enzymes; Germination
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-009-0102-y

To understand the adaptability of alfalfa ( Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at 10°C. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.